α-L-Fucosidase Activity Assay Kit II
A simple, fast and sensitive fluorometric assay to quantitate α-L-Fucosidase (FUCA1) in a variety of samples.
PromoCell’s α-L-Fucosidase Activity Assay Kit II provides a simple, sensitive and high-throughput adaptable approach to detect physiological concentrations of this glycosidase in a variety of biological samples. In this assay, FUCA1 uses a synthetic 4-MUF substrate and releases a fluorescent methylumbelliferyl derivative (4MU) that can be measured kinetically under acidic conditions (Ex/Em = 330/450 nm). The assay is a single-step reaction, with minimal sample preparation and can detect less than 1 µU/ml of FUCA1 activity in serum samples.