Technical library - RESOURCES

Tumor-associated macrophages (TAM) represent an important constituent of the tumor microenvironment. After being educated by cancer cells, TAM adopt an anti-inflammatory, pro-tumor and pro-metastatic M2-like phenotype.

Our Primary Cancer Cell Medium D-ACF allows for the isolation and culture of tumor associated macrophages (TAM) from solid tumors while reliably preventing stromal overgrowth.

The isolated TAM are non-proliferating adherent cells and can be cultured for at least two weeks.

The NCCD (Non-Cancerous Cell Depletion) reagent is a non-cytotoxic and functional component of the PCCS - necessary for the selective maintenance of malignant cells.

It should be used throughout the whole culture in our PCCS, even for suspension cultures.

No, the supplied serum is not heat-inactivated.

In principle, both types of liquid nitrogen storage are acceptable, each having its advantages and disadvantages.

Liquid phase storage provides a consistent temperature of -196°C, a longer holding time and a greater vial capacity but involves the risk of contamination issues.
Storage in the gas phase is very safe with respect to contaminations but the holding time of the cells is shorter and the vial capacity is reduced.

Our chondrocytes are derived from patients (~55-80 years) who underwent surgery for total endoprothesis of the hip or knee joint. In most cases this is necessary due to arthrosis. If the tissue shows macroscopic lesions, it is not used for cell isolation.

The amount of media needed per vial depends on the growth characteristics of the cells, the size of the TC vessels and the split ratios used, the frequency of media changes, the type of experiments you perform, etc. It is therefore difficult to give definite quantities. As a rough guideline, 1-2 bottles (500 ml each) are needed for 1 vial of HUVEC.

PromoCell's Osteoblast Basal Medium is an optimized media formulation developed for human osteoblast culture. The exact composition is proprietary.

Generally, you can split differentiated adipocytes. But these cells lose their ability to proliferate after differentiation and you can't expand the culture any more. Therefore it is recommended to plate the cells into the needed vessels (e.g. multiwell plates) prior to induction of differentiation so that trypsinization isn't necessary.

HFDPC are isolated from the hair papilla of normal human scalp hair follicles. Hair papilla in the adult hair follicle play a crucial role in the dermal-epidermal interactions that control hair production and in hair growth cycle events. The follicle dermal cells are cryopreserved at second passage and can be cultured for at least 10 population doublings when using PromoCell Follicle Dermal Papilla Cell Growth Medium (Cat. C-26501). Typical population doubling times are between 20-36 hrs. The recommended seeding density after thawing/trypsinization is 5,000-10,000 cells/cm². Using 1:4 splits, you can perform 4-5 passages with the cells.

Typical cell densities are between 32,000 - 40,000 cells/cm² (approx. 800,000 - 1 million cells per T25-flask).

If you purchase a proliferating culture of human osteoblasts (C-12760), there will be > 500,000 cells in the TC-flask. Once the culture is subconfluent, you will count between 750,000 - 1.1 million cells/T25 (depending on the cell lot).

Our Osteoblast Growth Medium (C-27001) consists of the Basal Medium supplemented with 10% (v/v) FCS, but with no recombinant growth factors. The medium primarily supports the proliferative capacity of normal human osteoblasts. It does not contain osteogenic factors (like dexamethasone and beta-glycerophosphate) that promote differentiation, as many users test their own chemical compounds (growth factors, hormones), or examine the effects of physical strain or sheer stress on the differentiated functions.

The Osteoblast Growth Medium is well-suited as the basis for these applications and can be supplemented with further growth factors if necessary. To specifically induce mineralization, we supply Osteoblast Mineralization Medium (C-27020).