How many passages can I perform with PromoCell HUVECs?

PromoCell guarantees more than 15 population doublings (PDs) for its HUVECs.
The number of passages that can be performed depends on the split (dilution) ratio used during subculture:

• With a 1:4 split ratio, HUVECs typically achieve ~2 population doublings per passage
• Under these conditions, HUVECs can usually be cultured for at least 6–8 passages

Please note that passage number alone is not a fixed parameter, as it varies with the chosen split ratio and culture conditions. Population doublings are the more accurate measure of cellular age.