Search results for: 'supplements'

Both cytokines are produced in E.coli.

Supplement Mix for Melanocyte Growth Medium M2 (C-39420) is free of serum and doesn't contain any PMA. The qualitiative and quantitative composition is proprietary.

Note: Melanocyte Growth Medium M2 and NHEM M2 cells were disontinued at the end of 2024.

PromoCell's Endothelial Cell Growth Medium (C-22010) is a complete medium that can be used for the culture of HUVEC after addition of the SupplementMix. Addition of extra FCS is not necessary. The SupplementMix contains FCS (2% v/v final concentration); recombinant growth factors; hormones; and a bovine brain extract that together have mitogenic effects on endothelial cells.

After addition of the SupplementMix or SupplementPack to our basal medium do I have to add FCS you obtain the complete growth medium. No further supplementation with serum or growth factors is required. Please note: Our media do not contain antibiotics. If you wish to use antibiotics you can add penicillin/streptomycin or gentamicin/amphotericin B at standard concentrations. Addition of antibiotics can however reduce the doubling time of the cells up to 30-40%.

Yes you can aliquot the SupplementMix upon delivery and freeze down 2 or 4 individual aliquots at -20°C. This way you can prepare smaller volumes (2 x 250 ml or 4 x 125 ml) of complete culture medium and thus extend the time you can use the medium.

Upon arrival the basal media should be stored between 4°C and 8°C the supplements at -20°C.

The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified; the composition of the supplements is confidential.

PromoCell Growth Media have special formulations and are much more complex than DMEM or RPMI + FBS. In comparison to immortalized cell lines primary cells have much higher nutrient and growth factor requirements. Classical media do not usually achieve good performances with our cells.

Our Osteoblast Supplement consists of FCS (10 % [v/v] final concentration) which is specifically tested to support optimal growth of Normal Human Osteoblasts.

The PromoCell Basal Media must be stored between 4-8°C and should not be frozen; as this can lead to precipitations. The same is true after addition of the supplements: the complete medium has to be kept at 4-8°C. If you prefer to make up smaller volumes of complete medium; you can aliquot the Supplement Mix and refreeze those aliquots at -20°C until use. This way you can extend the period in which you can use the supplemented media.

The Chondrocyte Growth Medium SupplementMix consists of Fetal Calf Serum (final concentration 10 % v/v) which has specifically been tested for the culture of primary chondrocytes. FCS contains a variety of different growth factors which are however not analyzed in more detail. There are no further growth factors added to the SupplementMix.

Light flocculation may be seen upon thawing the supplements containing ECGS/heparin or BPE. This does not affect the activity of our media. Optionally; the precipitate can be removed by centrifugation under sterile conditions. We recommend to thaw the supplements (SupplementMix or SupplementPack) at 15-25°C.