Following the recommended seeding density for your M1 macrophages (100,000 cells per cm2), I would need too many cells to have a complete 96- or 384-well plate. This is too expensive for me, can I reduce the number of cells per cm2?
Description
The recommended seeding density with 100,000 cells per cm2is needed for a confluent cell layer as the cells do not proliferate. However, you can reduce the seeding density by the factor 3 to 5 and the macrophages are still viable.