What antibiotic concentrations should I add to PromoCell media?

For optimal cell performance, we generally recommend culturing cells without antibiotics whenever possible. However, if the use of antibiotics is deemed necessary, please use the following final concentrations:
Penicillin: 100 U/ml + Streptomycin: 100 µg/ml
or
Gentamicin: 50 µg/ml + Amphotericin B: 50 ng/ml
 
Important note
The addition of antibiotics may reduce the growth rate of cells and can mask low‑level contamination. Antibiotics should therefore be used with caution, but may be helpful in situations where maintaining contamination‑free cultures is particularly challenging.