Are there any components in your Cancer Culture Media or in the coating reagent NCCD that could inhibit important proteins or signaling pathways?

While we cannot disclose the full formulation details, we can confirm that the media in our Cancer Media Toolbox contain standard cell culture components, such as salts, amino acids, vitamins, and growth supplements. They do not contain synthetic inhibitors, including ROCK inhibitors, protease inhibitors, or other pathway-specific inhibitors.
The Primary Cancer Culture System (PCCS), including the NCCD coating reagent, promotes the selective expansion of cancer stem cells (CSCs) by providing a growth advantage rather than by actively suppressing or eliminating other cell populations. Consequently, non-CSCs may become depleted over time as CSCs are preferentially supported, but this is not caused by active inhibition of specific cell types, proteins, or signaling pathways.
Please note: In Cancer Cell Line Medium XF (CCLM XF) and 3D Tumorsphere Medium XF, the only undefined component is human serum albumin (HSA) derived from human plasma.