How homogeneous is the MSC population, and do all cells differentiate into osteoblasts when cultured in MSC Osteogenic Differentiation Medium (C‑28013)?

Human MSCs are a heterogeneous population, and not all cells will differentiate into osteoblasts.
Differentiation efficiency depends strongly on the tissue source:

• hMSC‑BM: very good osteogenic differentiation
• hMSC‑AT: very good osteogenic and adipogenic differentiation
• hMSC‑UC: weak osteogenic differentiation, mainly chondrogenic

To obtain a high percentage of osteoblast‑like cells, hMSC‑BM or hMSC‑AT are the preferred sources. Please note that lot‑to‑lot variability exists, and differentiation capacity decreases with increasing passage number.
If particularly strong osteogenic differentiation is required, PromoCell Scientific Support can assist with lot selection prior to ordering.