Which DC Generation Medium does PromoCell recommend for freshly isolated MNC or monocytes, and which for cryopreserved monocytes?
PromoCell offers two different DC Generation Media, optimized for different starting materials.
Freshly isolated mononuclear cells (MNC) or monocytes
For freshly isolated MNC or monocytes, PromoCell recommends DC Generation Medium XF (C‑28052). This serum‑free and xeno‑free medium is optimized for the direct differentiation of monocytes into immature and mature myeloid dendritic cells.
Cryopreserved monocytes
For cryopreserved monocytes, PromoCell recommends DC Generation Medium (C‑28050).
This medium is optimized to support dendritic cell generation after thawing of monocytes.
Important note for fresh MNC with DC Generation Medium (C‑28050)
If DC Generation Medium (C‑28050) is used with freshly isolated MNC, an additional initial step is required:
Monocyte Attachment Medium (C‑28051) must be used first to ensure efficient adherence and enrichment of the monocyte fraction.
Further details are provided in the Instruction Manual and the corresponding Application Note: Generation of monocyte-derived Dendritic Cells (moDCs).
Freshly isolated mononuclear cells (MNC) or monocytes
For freshly isolated MNC or monocytes, PromoCell recommends DC Generation Medium XF (C‑28052). This serum‑free and xeno‑free medium is optimized for the direct differentiation of monocytes into immature and mature myeloid dendritic cells.
Cryopreserved monocytes
For cryopreserved monocytes, PromoCell recommends DC Generation Medium (C‑28050).
This medium is optimized to support dendritic cell generation after thawing of monocytes.
Important note for fresh MNC with DC Generation Medium (C‑28050)
If DC Generation Medium (C‑28050) is used with freshly isolated MNC, an additional initial step is required:
Monocyte Attachment Medium (C‑28051) must be used first to ensure efficient adherence and enrichment of the monocyte fraction.
Further details are provided in the Instruction Manual and the corresponding Application Note: Generation of monocyte-derived Dendritic Cells (moDCs).
Products relevant to this topic
Human CD14+ Monocytes (hMoCD14+-PB)
Cat No
C-12909
Human CD14+ Monocytes isolated from peripheral blood (hMoCD14+-PB) of single donors.
DC Generation Medium (Ready-to-use)
Cat No
C-28050
Cell culture medium for the generation of dendritic cells from peripheral blood monocytes.
DC Generation Medium XF (Ready-to-use)
Cat No
C-28052
Serum-free and xeno-free medium for the generation of dendritic cells from peripheral blood monocytes. The Base Medium XF does not include cytokines.
Monocyte Attachment Medium
Cat No
C-28051
Defined, animal-component free, and protein-free medium for efficient adherence selection of monocytes from freshly isolated human mononuclear cells.