What is the source of PromoCell Normal Human Follicle Dermal Papilla Cells (HFDPC), and how long can they be maintained in culture?
PromoCell Normal Human Follicle Dermal Papilla Cells (HFDPC) are isolated from the hair papilla of normal human scalp hair follicles. Dermal papilla cells in adult hair follicles play a key role in dermal-epidermal interactions, which regulate:
- hair production, and
- hair growth cycle events.
- HFDPC are cryopreserved at passage 2.
- When cultured in PromoCell Follicle Dermal Papilla Cell Growth Medium (C‑26501), they can be propagated for at least 10 population doublings
- Typical population doubling times: approximately 20–36 hours
- Recommended seeding density (after thawing or trypsinization): 5,000-10,000 cells/cm²
- Using a 1:4 split ratio, 4-5 passages can typically be performed.
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