Can PromoCell HUVECs be subjected to starvation?
Yes. Many customers perform serum starvation experiments with PromoCell HUVECs.
Most commonly, Endothelial Cell Basal Medium supplemented with FBS, but without any additional growth supplements, is used as the starvation medium. Typical conditions include:
Most commonly, Endothelial Cell Basal Medium supplemented with FBS, but without any additional growth supplements, is used as the starvation medium. Typical conditions include:
- 0.5–1% FBS for short-term serum starvation
- 5–20% FBS for longer incubation periods (e.g. 24-48 hours). Although these conditions are not serum-reduced, cells remain deprived of the growth factors normally present in Endothelial Cell Growth Medium and can therefore be considered growth factor-starved.
- Cells should be in good physiological condition before starting starvation.
- Experiments should be terminated shortly after the starvation period.
- Prolonged starvation can induce apoptosis and compromise cell viability.
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