Are airway epithelial cells other than HBEpC compatible with the PromoCell ALI Medium?
This depends on the specific airway epithelial cell type.
HNEpC and HTEpC
Yes, these cell types are compatible with PromoCell ALI Medium.
Please refer to the Application Note "Air-Liquid Interface Culture of Nasal and Tracheal Airway Epithelial Cells" that specifically describes the differentiation of HNEpC and HTEpC at the air-liquid interface (ALI). It provides the recommended conditions for successful ALI differentiation of these cell types.
HSAEpC
No. PromoCell ALI Medium is not suitable for differentiation of HSAEpC at the air-liquid interface.
Therefore, no ALI differentiation protocol is provided for this cell type.
HBEpC
For Human Bronchial Epithelial Cells (HBEpC), PromoCell offers special ALI pre‑screened lots.
These lots have been successfully tested for barrier function as part of PromoCell’s quality control. For availability and detailed information on ALI pre‑screened HBEpC lots, please contact PromoCell Scientific Support.
HNEpC and HTEpC
Yes, these cell types are compatible with PromoCell ALI Medium.
Please refer to the Application Note "Air-Liquid Interface Culture of Nasal and Tracheal Airway Epithelial Cells" that specifically describes the differentiation of HNEpC and HTEpC at the air-liquid interface (ALI). It provides the recommended conditions for successful ALI differentiation of these cell types.
HSAEpC
No. PromoCell ALI Medium is not suitable for differentiation of HSAEpC at the air-liquid interface.
Therefore, no ALI differentiation protocol is provided for this cell type.
HBEpC
For Human Bronchial Epithelial Cells (HBEpC), PromoCell offers special ALI pre‑screened lots.
These lots have been successfully tested for barrier function as part of PromoCell’s quality control. For availability and detailed information on ALI pre‑screened HBEpC lots, please contact PromoCell Scientific Support.
Products relevant to this topic
Human Nasal Epithelial Cells (HNEpC)
Cat No
C-12620
Primary Human Nasal Epithelial Cells isolated from normal human nasal mucosa.
Human Bronchial Epithelial Cells (HBEpC)
Cat No
C-12640
Primary Human Bronchial Epithelial Cells isolated from the surface epithelium of human bronchi.
Human Small Airway Epithelial Cells (HSAEpC)
Cat No
C-12642
Primary Human Small Airway Epithelial Cells isolated from the human distal respiratory tract.
Human Tracheal Epithelial Cells (HTEpC)
Cat No
C-12644
Primary Human Tracheal Epithelial Cells isolated from surface epithelium of human trachea.
Air-Liquid Interface Medium
Cat No
C-21080
Serum-free and BPE-free cell culture medium for an optimal and standardized culture of human bronchial epithelial cells at the air-liquid interface (ALI).