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Application Notes

Items 1-12 of 42 Results

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  • Human white preadipocyte differentiation

    Human white preadipocyte differentiation

    This application note provides a detailed protocol for the differentiation of preadipocytes into mature adipocytes.
  • Isolation and expansion of primary airway epithelial cells

    Isolation and expansion of primary airway epithelial cells

    Get a detailed protocol, along with insights into cell types, applications, physiological relevance, and common challenges.
  • Generation of human airway organoids from primary cells

    Generation of human airway organoids from primary cells

    This application note describes the generation of functional 3D airway organoids with apical-out or inward-oriented cilia formation based on human bronchial epithelial cells (HBEpC) as an in vitro model. These organoids can be used for high-throughput drug screening or large-scale virus-host interaction studies.
  • Long-term cultivation of melanocytes using serum- and BPE-free Melanocyte Growth Medium M3 (prf)

    Long-term cultivation of melanocytes using serum- and BPE-free Melanocyte Growth Medium M3 (prf)

    This application note describes the cultivation of melanocytes in PromoCell's clinically upgradable medium that ensures excellent growth performance for primary and adult skin cells, outperforming competitor formulations.
  • Air-Liquid Interface Culture System for standardized respiratory research: A serum- and BPE-free system

    Air-Liquid Interface Culture System for standardized respiratory research: A serum- and BPE-free system

    Generation of a 3D human airway model with primary human bronchial epithelial cells using the PromoCell serum- and BPE-free Air-Liquid Interface Culture System.
  • Air-Liquid Interface Culture of Nasal and Tracheal Airway Epithelial Cells

    Air-Liquid Interface Culture of Nasal and Tracheal Airway Epithelial Cells

    Generation of a 3D human airway model with primary human nasal and tracheal epithelial cells using our Air-Liquid Interface Medium (ALI-Airway).
  • Expansion of primitive Hematopoietic Progenitor Cells

    Expansion of primitive Hematopoietic Progenitor Cells

    Researchers are facing difficulties during in vitro expansion of undifferentiated hematopoietic stem cells (HSC). Our Hematopoietic Progenitor Cell (HPC) Expansion Medium XF provides a highly optimized, and xeno-free culture system for human HSC.
  • Real-time analysis of stem cell proliferation during differentiation

    Real-time analysis of stem cell proliferation during differentiation

    Here we describe the real-time analysis of human stem cell differentiation using cell-based assays. This allows for quantification of cell viability, proliferation and apoptosis.
  • Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells

    Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells

    Detailed analysis of human stem cells offers the potential for the specific treatment of diseases by cell-based therapies. With an automated real-time analysis, we assess phenotypic changes during mesenchymal stem cell (MSC) differentiation.
  • Osteogenic differentiation and analysis of MSC thumbnail

    Osteogenic differentiation and analysis of mesenchymal stem cells (MSC)

    Differentiate mesenchymal stem cells (MSC) into osteoblasts. Read our application note and find out more. The multipotency of these cells is one essential characteristic of MSC, according to the International Society for Cellular Therapy.
  • Neurogenic differentiation and analysis of mesenchymal stem cells (MSC)

    Neurogenic differentiation and analysis of mesenchymal stem cells (MSC)

    Specific culture conditions allow for transdifferentiation of mesenchymal stem cells (MSC) into cells of non-mesenchymal origin such as neurons. To do so, specific culture conditions and stimuli are needed.
  • Chondrogenic differentiation and analysis of mesenchymal stem cells (MSC)

    Chondrogenic differentiation and analysis of mesenchymal stem cells (MSC)

    Managed in vitro differentiation of mesenchymal stem cells (MSC) into chondrocytes. Multipotent adult stem cells are important for various tissues of the musculoskeletal system.
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