My Normal Human Cells are growing slowly after subculture. What could be the reason?
Slow growth after subculture is often caused by over‑trypsinization, which can damage the cells and reduce their viability.
In addition, suboptimal culture or handling conditions may also affect growth, for example:
Insufficient inactivation of trypsin
EDTA not removed by centrifugation after detachment
Incorrect seeding density
Use of non‑recommended media or supplements
To ensure optimal growth, please follow the recommended subculture protocol and culture conditions provided in the Product Manual.
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