How should I best freeze normal human cells?

Q: How should I best freeze normal human cells?

Cell Freezing Protocol : Detach the cells using your standard procedure. Count the cells and ensure a viability of >70%. Centrifuge and resuspend the cells in a suitable freezing medium at a density of 1-4×10⁶ cells/ml. - We recommend using Cryo‑SFM Plus , a defined, animal‑component‑free and protein‑free cryopreservation medium. - The freezing medium should be pre‑cooled to 4°C; room temperature (RT) should not be exceeded. Cool the cells slowly to -80°C at an approximate rate of -1°C per minute. - We recommend using “Mr. Frosty” (Nalgene), which ensures gradual and controlled cooling when placed in a -80°C freezer overnight. Transfer the vials to liquid nitrogen for long‑term storage.

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Cell Freezing Protocol:

Detach the cells using your standard procedure.
Count the cells and ensure a viability of >70%.
Centrifuge and resuspend the cells in a suitable freezing medium at a density of 1-4×10⁶ cells/ml.
- We recommend using Cryo‑SFM Plus, a defined, animal‑component‑free and protein‑free cryopreservation medium.
- The freezing medium should be pre‑cooled to 4°C; room temperature (RT) should not be exceeded.
Cool the cells slowly to -80°C at an approximate rate of -1°C per minute.
- We recommend using “Mr. Frosty” (Nalgene), which ensures gradual and controlled cooling when placed in a -80°C freezer overnight.
Transfer the vials to liquid nitrogen for long‑term storage.

How should I best freeze normal human cells?

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