How do I best isolate RNA from PromoCell cell pellets (C-14**)?
There are two options for isolating RNA from cells stored in RNAlater Solution: 1) The solution is removed from the cells prior to extraction by centrifugation at 5,000 x g for 10 minutes at 4°C. Note: Because of the density of RNAlater® solution, greater centrifugal forces are required to spin down the cells. 2) If no pellet is visible after centrifugation, RNA can also be purified directly from the RNAlater® solution. This can be done by adding 2 ml of 10x lysis buffer, and proceeding normally.
Products relevant to this topic
Human Follicle Dermal Papilla Cells (HFDPC), Cell Pellet in RNAlater
Cat No
C-14005
Cell pellet of HFDPC from human dermis of the scalp in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Umbilical Artery Endothelial Cells (HUAEC), Cell Pellet in RNAlater
Cat No
C-14013
Cell pellet of HUAEC from the arteries of the umbilical cord in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Coronary Artery Endothelial Cells (HCAEC), Cell Pellet in RNAlater
Cat No
C-14022
Cell pellet of HCAEC from human coronary arteries of single donors in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Aortic Endothelial Cells (HAoEC), Cell Pellet in RNAlater
Cat No
C-14023
Cell pellet of HAoEC from the human aorta in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Pulmonary Artery Endothelial Cells (HPAEC), Cell Pellet in RNAlater
Cat No
C-14024
Cell pellet for HPAEC from human pulmonary arteries in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Saphenous Vein Endothelial Cells (HSaVEC), Cell Pellet in RNAlater
Cat No
C-14025
Cell pellet of HSaVEC from saphenous veins from single donors in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Pulmonary Microvascular Endothelial Cells (HPMEC), Cell Pellet in RNAlater
Cat No
C-14027
Cell pellet of HPMEC from the lung from single donors in RNAlater® for subsequent RNA, DNA or protein analysis.
Human Uterine Microvascular Endothelial Cells (HUtMEC), Cell Pellet in RNAlater
Cat No
C-14028
Cell pellet of HUtMEC from the myometrium in RNAlater® for subsequent RNA, DNA or protein analysis.