Can PromoCell Human Stem Cells, Pericytes, and Blood Cells be re-frozen?
The suitability for re-freezing depends on the cell type.
- Human Mesenchymal Stem Cells (hMSC) and human placental pericytes (hPC‑PL) can be re-frozen. However, each additional freeze-thaw cycle may reduce cell recovery, viability, and proliferation capacity. As a result, the number of population doublings (PDs) specified in the Certificate of Analysis (CoA) can no longer be guaranteed. If re-freezing is required, we recommend doing so at a low passage number using Cryo‑SFM Plus (C‑29920), a defined, animal component-free, and protein-free cryopreservation medium.
- Human Mononuclear Cells (hMNC) and CD14⁺ monocytes can technically be re-frozen, but substantial cell damage and reduced recovery should be expected. Since these cells do not proliferate, re-freezing is generally not recommended.
- M1 and M2 macrophages should not be re-frozen.
Products relevant to this topic
Cryo-SFM Plus, 30 ml
Cat No
C-29920
Defined, animal-component free, and protein-free cryopreservation medium for optimal cell storage.
Human Mononuclear Cells from Peripheral Blood (hMNC-PB), single donor, ultra-pure
Cat No
C-12907
Primary Human Mononuclear Cells isolated from umbilical cord blood and peripheral blood.
Human CD14+ Monocytes (hMoCD14+-PB)
Cat No
C-12909
Human CD14+ Monocytes isolated from peripheral blood (hMoCD14+-PB) of single donors.
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12914
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium XF (incl. GM-CSF).
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12915
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).
Human CD34+ Progenitor Cells (hCD34+-CB)
Cat No
C-12921
Human CD34+ Progenitor Cells from Cord Blood (hCD34+ -CB) of a single donor.
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
Cat No
C-12971
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.