Airway Epithelial Cell Growth Medium XF (prf)
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Xeno- and serum-free cell culture medium for epithelial cells isolated from large air passages.
Cat No
C-21050
Unit
477.00 CHF
Original price:
Product description
Airway Epithelial Cell Growth Medium XF is a xeno-, serum-, and phenol red-free formulation optimized for the in vitro cultivation of epithelial cells from large air passages, including HBEpC, HNEpC, and HTEpC.
Key features
- Optimized for in vitro cultivation of epithelial cells from large air passages (HBEpC, HNEpC, HTEpC)
- Xeno-free and serum-free
- Phenol red-free
- Supplied as a 500 ml bottle of Airway Epithelial Cell Basal Medium XF (prf) with premixed SupplementMix
- Average population doubling time (including lag phase) over 3 passages ≤70 h
Product benefits
- Physiologically relevant growth: Supports cell growth, cobblestone morphology, and airway-specific functions that closely mimic the in vivo environment without the need for small-molecule additives.
- Regulatory compliance: Xeno-free composition ensures batch-to-batch uniformity, reducing variability and improving reproducibility—ideal for large-scale manufacturing.
- Phenol red-free formulation: Eliminates non-functional ingredients for sensitive applications
- NO compromise in cell growth: Achieves equal or greater population doublings compared to BPE-containing and serum-free media.
- Supports primary cell isolation: Proven for isolation of HBEpC, HNEpC, and HTEpC from fresh bronchial, nasal, and tracheal tissues without additional medium components.
- Proven 3D culture performance: Supports the formation of functional airway organoids—rotating spheroid-like structures with predominantly apical-out cilia—for ≥28 days in basal membrane extract.
Data & figures
Figure 1.
Typical small and homogenous cobblestone morphology of HBEpC in our xeno-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 2.
Maintenance of typical cobblestone morphology in highly proliferative HNEpC in our xeno-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 3.
HTEpC isolated and expanded in xeno-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 4.
Increased cell yield of isolated HNEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 5.
Growth curve of primary human HBEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 6.
Growth curve of primary human HTEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 7.
Growth curve of primary human HNEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium XF (prf).
Figure 8.
Cell identity of isolated HTEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium XF (prf).
Resources
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