Tumorsphere cell culture: Method and efficiency

Read about the tumorsphere culture and the tumorsphere formation efficiency, including protocols.

Cancer stem cells (CSCs) can be enriched and cultured as extended serial 3D tumorsphere cultures, thus permitting the establishment of a more physiological 3D microenvironment.

In this poster, you will get

  • List of cancer cell types to establish successful 3D tumorsphere culture
  • 3D Tumorosphere culture protocol
  • Tumorsphere formation efficiency (TFE) Assay; detailed protocol including quantification process

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