Should cryopreserved cells be stored in the liquid phase or the gas phase of liquid nitrogen?
In principle, both liquid‑phase and gas‑phase storage of cryopreserved cells in liquid nitrogen are acceptable. Each option has specific advantages and limitations.
Liquid phase storage
Liquid phase storage
- Provides a constant temperature of −196°C
- Allows for a longer storage time and higher vial capacity
- However, it poses a risk of contamination.
- Considered very safe with regard to contamination
- Temperature is slightly higher and less uniform than in the liquid phase
- Shorter holding time and reduced vial capacity compared to liquid‑phase storage
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