How does PromoCell ensure that pericyte cultures are free of fibroblast contamination? Are fibroblasts removed using anti‑CD90 magnetic beads?
No, PromoCell does not perform CD90‑based immunomagnetic separation for pericytes.
However, fibroblast contamination can be reliably excluded based on the following criteria:
How fibroblast contamination is ruled out
1. Growth behavior after isolation
- Pericytes require up to 2 weeks before they start proliferating.
- Fibroblasts, in contrast, proliferate immediately after isolation and would rapidly overgrow the culture.
- Any fibroblast contamination would therefore be clearly detectable shortly after isolation.
2. Phenotypic characterization during Quality Control
- Pericytes are characterized by flow cytometry for CD146 expression.
- Pericytes express CD146, whereas placental fibroblasts do not.
- This marker analysis confirms the identity and purity of the pericyte population.
