Why is the thawing protocol different for cryopreserved macrophages?
Cryopreserved human macrophages require a specialized thawing protocol because they are particularly sensitive to freeze-thaw stress.
During the recovery phase, the cells are incubated in cold medium (2-8°C) for 20 minutes before seeding. This gradual warming process allows the cells time to recover and restore membrane integrity, resulting in improved cell viability and attachment.
Based on our experience, this recovery step significantly enhances the adhesion efficiency of cryopreserved human macrophages after thawing.
Please note: For detailed thawing instructions, please refer to the corresponding Instruction Manual.
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