I would like to differentiate hMSC into bone cells and further maintain the cells in culture after differentiation. Which medium should I use?

Osteogenic differentiation of hMSCs with PromoCell MSC Osteogenic Differentiation Medium takes ∼14 days (please view the respective Application Note or Product Manual for a detailed protocol).

Use collagen- or fibronectin-coated plates and change the medium every third day. The bone cells tend to detach from the plastic after approximately 2 weeks, when differentiation is complete. Therefore, the tests should be performed promptly and the cells should be maintained in MSC Osteogenic Differentiation Medium until then.