How long do I need to activate my macrophages, and can I stop adding activation factors after 24 hours?
The activation of macrophages is generally complete after 24 hours.
However, if you wish to maintain the activated state over a longer period of time (e.g. several days), fresh activation factors should be added with every medium change.
Stopping the addition of activation factors after 24 hours may lead to a gradual loss of the activation status during prolonged culture.
However, if you wish to maintain the activated state over a longer period of time (e.g. several days), fresh activation factors should be added with every medium change.
Stopping the addition of activation factors after 24 hours may lead to a gradual loss of the activation status during prolonged culture.
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